ABSTRACT
Objective:To investigate the effects and its mechanism of lipopolysaccharide on the proliferation,differentiation and apoptosis of human dental pulp stem cells by Notch signaling pathway. Methods:Human dental pulp stem cells( hDPSCs) was isolated from dental pulp tissue;cell proliferation was detected after 0,0. 1,1,10 μg/ml treated cells for 1,3,5,7 days by CCK8 test;related mRNA expression ALP,DSPP,DMP1 gene was detected after 1 μg/ml lipopolysaccharide treated hDPSCs for 0,3,7,14,21 day by RT-PCR;cell apoptosis was detected after 1 μg/ml lipopolysaccharide treated hDPSCs for 0,7,14,21 day by flow cytometry. Cleaved caspase3,Notch1,Hes1 protein expression was detected by Western blot. Results:Cell proliferation after different concentrations lipopo-lysaccharide stimulated hDPSCs for 1, 3, 5 days had no significant difference, significantly lower at 7 day than 0 μg/ml lipopolysaccharide group(P<0. 01). ALP,DSPP,DMP1 mRNA expression lipopolysaccharide treated hDPSCs at 3 day compared with the control group had no statistical significance(P>0. 05),significantly higher at 7,14,21 day than control group(P<0. 01);cells apoptosis rate and Cleaved caspase3,Notch1,Hes1 protein expression lipopolysaccharide treatment hDPSCs at 7,14 and 21day was sig-nificantly higher than the control group(P<0. 01);ALP,DSPP,DMP1 mRNA expression and apoptosis rate and Cleaved caspase3, Notch1,Hes1 protein expression at 21 day was a downward trend. Conclusion:Lipopolysaccharide can decrease the proliferation of hDPSCs and promote its mineralization and apoptosis,which may be related to the activation of Notch signaling pathway.
ABSTRACT
Objective:To investigate the effects and its mechanism of lipopolysaccharide on the proliferation,differentiation and apoptosis of human dental pulp stem cells by Notch signaling pathway. Methods:Human dental pulp stem cells( hDPSCs) was isolated from dental pulp tissue;cell proliferation was detected after 0,0. 1,1,10 μg/ml treated cells for 1,3,5,7 days by CCK8 test;related mRNA expression ALP,DSPP,DMP1 gene was detected after 1 μg/ml lipopolysaccharide treated hDPSCs for 0,3,7,14,21 day by RT-PCR;cell apoptosis was detected after 1 μg/ml lipopolysaccharide treated hDPSCs for 0,7,14,21 day by flow cytometry. Cleaved caspase3,Notch1,Hes1 protein expression was detected by Western blot. Results:Cell proliferation after different concentrations lipopo-lysaccharide stimulated hDPSCs for 1, 3, 5 days had no significant difference, significantly lower at 7 day than 0 μg/ml lipopolysaccharide group(P<0. 01). ALP,DSPP,DMP1 mRNA expression lipopolysaccharide treated hDPSCs at 3 day compared with the control group had no statistical significance(P>0. 05),significantly higher at 7,14,21 day than control group(P<0. 01);cells apoptosis rate and Cleaved caspase3,Notch1,Hes1 protein expression lipopolysaccharide treatment hDPSCs at 7,14 and 21day was sig-nificantly higher than the control group(P<0. 01);ALP,DSPP,DMP1 mRNA expression and apoptosis rate and Cleaved caspase3, Notch1,Hes1 protein expression at 21 day was a downward trend. Conclusion:Lipopolysaccharide can decrease the proliferation of hDPSCs and promote its mineralization and apoptosis,which may be related to the activation of Notch signaling pathway.